Sub-acute toxicity services are a vital component of toxicology, focusing on evaluating the potential harmful effects of repeated, relatively short-term exposures to substances over a period ranging from 14 to 90 days. These services play a crucial role in assessing the safety and risk profiles of pharmaceuticals, chemicals, and environmental factors. They are instrumental in safety assessment, risk mitigation, and regulatory compliance, aiding in setting exposure limits, informing labeling requirements, and ensuring the safe introduction of new compounds into the market or environment. By conducting controlled experiments with animal models, monitoring various parameters, and analyzing dose-response relationships, sub-acute toxicity services provide essential data for decision-making in product development and environmental protection. Ethical considerations are paramount in conducting these studies, emphasizing the importance of minimizing harm to test subjects and using alternative methods when possible.